Preparation and properties of Pue-loaded HA-ADH-PS nanomicelles

Preparation and properties of Pue-loaded HA-ADH-PS nanomicelles

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Puerarin (Pue) is the most abundant isoflavonoid in kudzu root.It has been widely used as a therapeutic agent for the treatment of cardiovascular diseases.However, poor-bioavailability of puerarin is the main obstacle to its widespread clinical applications.In this paper, HA-ADH-PS nanomicelles were prepared by chemical modification, noncovalent modification and etc, and characterized by means of FT-IR, ultraviolet (UV) and thermogravimetric analysis (TG).The encapsulation efficiency and drug loading of Pue-loaded HA-ADH-PS nanomicelles were 45.

1% and 19.89% by UV, respectively.It could be observed from the transmission electron creta girl half wig microscopy (TEM) images that HA-ADH-PS micelles appeared obvious spherical structure in the water.The particle size of HA-ADH-PS nanomicelles and Pue-loaded HA-ADH-PS nanomicelles were about 136.8 nm and 119.

5 nm with a PDI of 0.237 and 0.272, respectively.The fluorescence probe method was used to characterize the critical micelle concentration, the critical micelle concentration (CMC) value of the nanomicells was 0.002 g/L and the results met the requirements and ensured the stability of micelles after dilution.

DPPH assay suggested that Pue-loaded HA-ADH-PS nanomicelles had an obvious radical scavenging effect in vitro.MTT test showed that Pue-loaded HA-ADH-PS nanomicelles was non-toxic and had good biocompatibility.Thus, Pue-loaded HA-ADH-PS nanomicelles barricini chocolate could be used as a potential drug copyright for puerarin.